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How the brain controls blood flow during sleep

- “Substance P” secreted by parvalbumin interneurons drive slow vasodilation -

Even while we are asleep, the brain does not rest completely. Surprisingly, the blood flow in a sleeping brain can be greater than when it is in a wakeful state. This allows the brain to remove waste metabolites, which is important to prevent the development and progression of neurological dysfunctions such as dementia. However, the exact mechanism of how the sleeping brain increases blood flow has not been known.

Researchers led by Director KIM Seong-Gi of the Center for Neuroscience Imaging Research within the Institute for Basic Science, South Korea, recently discovered the secrets behind this phenomenon. It was discovered that a type of inhibitory neuron called ‘parvalbumin (PV) neuron’ secretes material called ‘substance P’ that is responsible for vasodilation and control of blood flow to the brain.

Unlike other inhibitory subtype neurons, it was previously thought that GABAergic PV neurons do not release vasoactive substances, hence their role in blood flow regulation has been controversial. To investigate the role of PV neurons on blood flow regulation, the researchers expressed an opsin protein called channelrhodopsin-2 (ChR2) in mouse PV neurons, and selectively activated PV neurons by light stimulation. Vascular responses to the activation of PV neurons were measured by wide-field optical imaging and functional magnetic resonance imaging (fMRI). In addition, to identify the role of the PV neurons on blood flow under anesthesia, the researchers used ketamine/xylazine to put the animals to sleep.

The results showed that in lightly anesthetized animals, stimulation of PV neurons induced vasoconstriction and a decline in blood flow. This was followed by a slow vasodilation and an increase in blood flow from 20 seconds up to a minute after cessation of stimulation. On the other hand, in active animals, the PV neuron activity only resulted in a reduction of blood flow. This means that the PV neurons have two different mechanisms for the control of cerebral blood flow depending on whether the brain is awake or asleep.

Furthermore, the researchers also discovered the mechanism behind the observed ‘slow vasodilation after optogenetic stimulation. When the PV neurons are activated, it inhibits the nearby excitatory neurons, which causes vasoconstriction and reduced blood flow. At the same time, it was found that these PV neurons release a peptide called ‘substance P’, which is responsible for the observed slow vasodilation. Substance P activates cells called nitric oxide synthase (nNOS) GABAergic neurons secreting nitrous oxide, a known vasodilator.

The present research finally unveiled the factors that control blood flow to the brain during sleep, and the previously unknown role of PV neurons in this process. Director Kim said, “Our research suggested a new direction of research on cerebral blood flow control mechanisms, with possible implications in insomnia and sleep disorder treatment.”

This research was conducted jointly with Professor SUH Minah’s team at the SungKyunKwan University (SKKU) Department of Biomedical Engineering and Professor Patrick J. DREW of the Pennsylvania State University.


Figure 1. Role of PV neuron activity in the awake and anesthetized brain. When the inhibitory PV neuron is activated in the awake brain, excitatory neurons are inhibited and vasoconstriction is observed (left). In the anesthetized brain, substance P is secreted by the PV neurons, which activates nNOS neurons and causes slow vasodilation (right). Optical imaging was used to measure HbT (total hemoglobin), which is used to infer the level of blood flow.
Figure 1. Role of PV neuron activity in the awake and anesthetized brain. When the inhibitory PV neuron is activated in the awake brain, excitatory neurons are inhibited and vasoconstriction is observed (left). In the anesthetized brain, substance P is secreted by the PV neurons, which activates nNOS neurons and causes slow vasodilation (right). Optical imaging was used to measure HbT (total hemoglobin), which is used to infer the level of blood flow.


Figure 2. Researchers from the Center for Neuroscience Imaging Research. From left to right: Director KIM Seong-Gi (corresponding author), Thanh Tan VO (1st author), and IM Geun Ho (researcher).
Figure 2. Researchers from the Center for Neuroscience Imaging Research. From left to right: Director KIM Seong-Gi (corresponding author), Thanh Tan VO (1st author), and IM Geun Ho (researcher).


Notes for editors

- Reference
Thanh Tan Vo, Geun Ho Im, Kayoung Han, Minah Suh, Patrick J. Drew, and Seong-Gi Kim. Parvalbumin interneuron activity drives fast inhibition-induced vasoconstriction followed by slow substance P-mediated vasodilation. PNAS. DOI: 10.1073/pnas.2220777120.


- Media Contact
For further information or to request media assistance, please contact Seong-Gi Kim at the Center for Neuroscience Imaging Research, Institute for Basic Science (IBS) (seonggikim@skku.edu) or William I. Suh at the IBS Public Relations Team (willisuh@ibs.re.kr).


- About the Institute for Basic Science (IBS)
IBS was founded in 2011 by the government of the Republic of Korea with the sole purpose of driving forward the development of basic science in South Korea. IBS has 4 research institutes and 33 research centers as of January 2023. There are eleven physics, three mathematics, five chemistry, nine life science, two earth science, and three interdisciplinary research centers.

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Last Update 2023-11-28 14:20